| 摘要 |
PROBLEM TO BE SOLVED: To provide nanoscale devices for analyzing features, such as a physical size of macromolecules and biomarkers along large genomic DNA molecules, the device carrying out such high throughput analysis in a massively parallel fashion.SOLUTION: There is provided nanofluidic devices capable of manipulating a single elongated macromolecule, comprising: a substrate comprising a surface and one or more fluidic nanochannel segments disposed substantially parallel to the surface, wherein at least one of the fluidic nanochannel segments is capable of containing and elongating at least a portion of a macromolecule residing within at least a portion of the fluidic nanochannel segment, and wherein each of the fluidic nanochannel segments has a cross-sectional dimension of less than about 1,000 nm and a length of at least about 10 nm; and a cap capable of covering at least a portion of at least one nanochannel segments, wherein at least a portion of the cap is permeable to soluble analytes. |
