发明名称 METHOD FOR QUANTIFYING HUMAN DNA USING AN INTERNAL CONTROL
摘要 The present invention relates to a method for quantifying and/or detecting one or more nucleic acids of a genome in a sample, wherein in an amplification reaction, (i) a first nucleic acid is amplified, the locus that is amplified is a multicopy locus (MCL) within the genome, wherein the locus shares at least 80% sequence identity to a sequence according to SEQ ID NO. 1 over a stretch of 80 base pairs, and wherein the multicopy locus has copies on at least two different chromosomes, (ii) a second nucleic acid that has been added as an internal control (IC) is also amplified, and (iii) the amount of amplification product from the amplification of the first nucleic acid is determined.
申请公布号 US2014147843(A1) 申请公布日期 2014.05.29
申请号 US201214000092 申请日期 2012.02.20
申请人 DI PASQUALE FRANCESCA;ENGEL HOLGER;STRAUSS SASCHA;THEL WELL NICOLA JO;QIAGEN MANCHESTER LIMITED;QIAGEN GMBH 发明人 DI PASQUALE FRANCESCA;ENGEL HOLGER;STRAUSS SASCHA;THEL WELL NICOLA JO
分类号 C12Q1/68 主分类号 C12Q1/68
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