| 摘要 |
The present invention relates to a novel method and to a kit for preparing nucleic-acid libraries, in particular for high-throughput sequencing. Said method is useful for simultaneously preparing multiple nucleic-acid libraries for sequencing, each library being characterized by a specific sequence of barcodes. In other words, instead of preparing, in parallel, a plurality of libraries that will be barcoded, the method of the invention enables the simultaneous preparation of a plurality of barcoded libraries by carrying out the step of preparing a single library. The inventor provides a means for inserting the barcodes at the beginning of the method for preparing the library. The libraries provided with the method described herein can be used for any high-throughput sequencing platform, such as the 454 Genome Sequencer, the Illumina Genome Analyzer, or the SoLiD platform. |
