| 摘要 |
FIELD: medicine.SUBSTANCE: invention relates to the field of medicine, namely to laboratory diagnostics, and can be applied for identification of the prostate-specific antigen (PSA) in liquid medium. For this purpose liquid medium interacts with a sensor, made in a form of a flat flexible cantilever. At least, one of the planes of the cantilever contains silicon dioxide and can reflect light irradiation. One of the planes is covered with bovine serum albumin. Another contains two layers, one of which is covalently bound with the surface of the cantilever, and the other contains chemically connected with the previous layer molecules of the antibody, specifically indentifying the prostate-specific antigen. After that, changes of the cantilever curve are determined by lightening the cantilever surface by a light beam and measuring deviation of the light beam, reflected form the cantilever surface. As a sensor applied is the cantilever, in which the layer, covalently bound with the surface of the cantilever, is made of 3-aminopropylsiltron.EFFECT: invention ensures carrying out qualitative and quantitative analysis of liquid media for the PSA content and increases sensitivity of PSA identification to 0,1 nanogram/millimetre.5 ex |
