| 摘要 |
FIELD: medicine.SUBSTANCE: claimed invention relates to the field of microbiology. Group of inventions includes a method and a system for determining quantity of cultivated microbial cells, such as E. coli, Ps.aeroginosa, or a mixture of microbial cells (for instance, for determination of the total number of microbes) in an analysed sample. The said method includes (i) bringing the analysed sample, which can include cultivated microbial cells in contact with, at least, one signal agent, capable of binding with the microbial cell membrane, with the said bringing in contact being carried out for the preliminarily specified first time period (T1), sufficient for integration of the signal agent into the microbial cell to such a level at which the signal, emitted by the sample, in fact reaches a plateau; (ii) removal of non-internalised signal agent from the said analysed sample; (iii) identification of the signal cultivated cells among the signal objects in the said sample, based on selection parameters, preliminarily determined for the said cultivated cells, during the second time period (T2), following the said first time period (T1), during which the said signal in fact remains at the level of the said plateau; and (iv) determination of a quantitative value, indicating the number of the cultivated cells in the analysed sample on the basis of the said selected objects.EFFECT: group of inventions makes it possible to obtain, in fact in real time, highly accurate quantitative value, representing content of microbial cells in the sample, which can be an equivalent of CFU.39 cl, 4 dwg, 3 tbl |
