| 摘要 |
FIELD: biotechnologies.SUBSTANCE: invention relates to the field of gene engineering, specifically, to development of test systems based on fluorescent probes, which may be used as substrates for phospholipases A1 and A2. The probe with the name 1-[3-(2,4-dinitrophenylamino)propanoyl]-2-[8-(9-anthryl)-7E-octenoyl)]-sn-glycero-3-phosphocholine represents an analogue of phosphatidyl choline witth modified fatty acid chains, one of which carries the fluorescent group, and the other one - grouping-suppressor of fluorescence. The fluophor-suppressor pair is: 9-anthryl vinyl - 2,4-dinitrophenyl. The produced probe is included into the test system to determine activity of A2 phospholipase, group IIA, which also includes a micellar matrix, a buffer solution, diglyme and A2 phospholipase of apitoxin as standard.EFFECT: invention makes it possible to determine activity of A2 phospholipase in blood serum in case of acute pathological processes, in tissue and cell extracts.2 cl, 3 dwg, 5 ex |
