发明名称 |
Un procedimiento para la determinación de un agonista colinérgico selectivo para un receptor alfa7 nicotínico |
摘要 |
Methods of inhibiting release of a proinflammatory cytokine from a macrophage are provided. The methods comprise treating the macrophage with a cholinergic agonist in an amount sufficient to decrease the amount of the proinflammatory cytokine that is released from the macrophage, wherein the cholinergic agonist is selective for an ±7 nicotinic receptor. Methods for inhibiting an inflammatory cytokine cascade in a patient are also provided. The methods comprise treating the patient with a cholinergic agonist in an amount sufficient to inhibit the inflammatory cytokine cascade, wherein the cholinergic agonist is selective for an ±7 nicotinic receptor. Methods for determining whether a compound is a cholinergic agonist reactive with an ±7 nicotinic receptor are also provided. The methods comprise determining whether the compound inhibits release of a proinflammatory cytokine from a mammalian cell. Additionally, methods for determining whether a compound is a cholinergic antagonist reactive with an ±7 nicotinic receptor are provided. These methods comprise determining whether the compound reduces the ability of a cholinergic agonist to inhibit the release of a proinflammatory cytokine from a mammalian cell. Oligonucleotides or mimetics capable of inhibiting attenuation of lipopolysaccharide-induced TNF release from a mammalian macrophage upon exposure of the macrophage to a cholinergic agonist are also provided. The oligonucleotides or mimetics consist essentially of a sequence greater than 5 nucleotides long that is complementary to an mRNA of an ±7 receptor. Additionally, methods of inhibiting attenuation of TNF release from a mammalian macrophage upon exposure of the macrophage to a cholinergic agonist are provided. These methods comprise treating the macrophage with the above-described oligonucleotide or mimetic. |
申请公布号 |
ES2462545(T3) |
申请公布日期 |
2014.05.23 |
申请号 |
ES20070020473T |
申请日期 |
2003.12.05 |
申请人 |
THE FEINSTEIN INSTITUTE FOR MEDICAL RESEARCH |
发明人 |
TRACEY, KEVIN;WANG, HONG |
分类号 |
G01N33/566;A61K31/00;A61K31/439;A61K31/444;A61K31/46;A61P1/00;A61P9/00;A61P11/00;A61P15/00;A61P29/00;A61P31/00;A61P33/00;A61P37/00;A61P43/00;G01N33/68;G01N33/94 |
主分类号 |
G01N33/566 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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