METHOD FOR PRODUCING CORDYCEPIC ACID BY MEANS OF LIQUID FERMENTATION OF CORDYCEPS MILITARIS

摘要

Provided is a method for producing cordycepic acid by means of the liquid fermentation of Cordyceps militaris, comprising the steps of: (1) inoculating strains of Cordyceps militaris into the PD liquid fermentation medium, activating the culturing of the strains, then transferring them to a seed fermentation medium and obtaining a seed solution from the seed culture; (2) inoculating the seed solution into a liquid fermentation medium and culturing for 20 - 30 h, then adding an expansin solution and further culturing for 96 - 144 h, isolating and obtaining Cordyceps militaris mycelium; (3) extracting intracellular cordycepic acid of Cordyceps militaris from the Cordyceps militaris mycelium. An expansin is used in the liquid fermentation production of cordycepic acid of Cordyceps militaris, which increases the fermentation yield of cordycepic acid.