| 摘要 |
The present invention relates to a cell line of lymphocytes comprisingγδT cells, composition and production method thereof, and medical use namely for the use in medicine, namely in cancer immunotherapy. The cell line comprise a sample of human peripheral blood Vδ1+γδT cells expressing functional natural cytotoxicity receptors (NCRs). These Vδ1+ NCR+ T lymphocytes can directly mediate killing of leukemia cell lines and chronic lymphocytic leukemia patient neoplastic cells. The present invention shows that human Vδ1+ NCR+ T cells can be differentiated and expanded from totalγδperipheral blood lymphocytes (PBLs), upon regular in vitro or ex vivo stimulation withγδTCR agonists andγc-family cytokines. This subset surprisingly expresses NKp30, NKp44 and NKp46, and high levels of Granzyme B that associate with highly enhanced cytotoxicity against lymphoid leukemias. |
