| 摘要 |
<p>A biological preparation method of ethyl (R)-4-cyano-hydroxybutanoate. The reaction system of enzymatic synthesis of ethyl (S)-4-chloro-3-hydroxybutanoate is properly treated, and recombinant halohydrin dehalogenase is subsequently added. Under the effect of recombinant halohydrin dehalogenase, ethyl (S)-4-chloro-3-hydroxybutanoate reacts with sodium cyanide to give a target product ethyl (R)-4-cyano-hydroxybutanoate, and the reaction takes place in an aqueous phase of pH 6-8. Ethyl (S)-4-chloro-3-hydroxybutanoate is prepared by asymmetric reduction reaction of ethyl 4-chloro-3-oxobutanoate under the presence of recombinant ketoreductase enzyme, a cofactor and a hydrogen donor, the hydrogen donor is isopropanol, and the asymmetric reduction reaction takes place in a mixed system of an aqueous phase buffer solution of pH 7.0-9.0 and toluene. In the present invention, the two reactions can share one reaction system, which can reduce the material and energy consumption, effectively reduce the cost, solve the problem of high material and energy consumption of a single-step enzyme method, and solve the problem of a coupled enzyme method that the product cannot be produced effectively due to mutual interference.</p> |
