| 摘要 |
The present invention relates to a method for producing fatty acid alkyl ester which comprises a method for immobilizing L62 lipase comprising: a step for fixing L62 lipase derived from Staphylococcus haemolyticus to a carrier by hydrophobic bond; and a step for cross linking the L62 lipase by reacting a cross linking agent with the L62 lipase fixed to the carrier and a method for prodicung fatty acid alkyl ester comprising a step for transesterifying oils and alcohol in reactive medium or a step for transesterifying fatty acid and alcohol in the reactive medium using the immobilized L62 lipase. According to the present invention, the L62 lipase is fixed to the carrier stably using the carrier as a formboard, thereby maintaining activity in process of synthesizing the fatty acid alkyl ester and having an effect in shortening processes since the stability to various organic solvents is maintained, and reducing the unit cost of production since the fatty acid alkyl ester is synthesized from the waste oil. [Reference numerals] (AA) MA-DVB resin;(BB) L62 lipase;(CC) Absorption;(DD) Glutaraldehyde;(EE) Cross link |
