发明名称 |
PROTEIN PRODUCTION METHOD |
摘要 |
The present invention relates to a method for integrating a gene fragment inserted between a pair of transposon sequences into a chromosome of a mammalian cell, comprising introducing at least one expression vector which comprises a gene fragment comprising a DNA encoding a protein of interest and also comprises a pair of transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; and a method for producing the protein of interest comprising suspension-culturing a suspension mammalian cell which produces the protein of interest; and an a suspension mammalian cell which expresses the protein of interest. |
申请公布号 |
EP2653540(A4) |
申请公布日期 |
2014.05.21 |
申请号 |
EP20110848424 |
申请日期 |
2011.12.14 |
申请人 |
INTER-UNIVERSITY RESEARCH INSTITUTE CORPORATION RESEARCH ORGANIZATION OF INFORMATION AND SYSTEMS;KYOWA HAKKO KIRIN CO., LTD. |
发明人 |
KAWAKAMI KOICHI;KUROKAWA MEGUMI;YAMAGUCHI KEINA;OGAWA RISA;TSUKAHARA MASAYOSHI;HAYASHI YOKO |
分类号 |
C12N15/09;C07K16/10;C07K16/24;C07K16/28;C12N5/10;C12N15/85;C12P21/02;C12P21/08 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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