发明名称 |
UK-2 BIOSYNTHETIC GENES AND METHOD FOR IMPROVING UK-2 PRODUCTIVITY USING THE SAME |
摘要 |
The genomic DNA of Streptoverticillium sp. 3-7, which produces UK-2, was analyzed to identify a region expected to be a UK-2 biosynthetic gene cluster. Moreover, by colony hybridization, DNAs in the region were successfully isolated. Further, the DNAs were used to prepare a strain in which the genes present in the region were disrupted. The strain was found not to produce UK-2. It was verified that the genomic region was the UK-2 biosynthetic gene cluster. Furthermore, Streptoverticillium sp. 3-7 was transformed by introduction of a vector in which the isolated UK-2 biosynthetic gene cluster was inserted. It was also found out that the UK-2 productivity by the transformant was improved about 10 to 60 times or more in comparison with that of the parental strain. Moreover, it was revealed that 2 copies of the UK-2 biosynthetic gene cluster were present per cell in these transformants, respectively. |
申请公布号 |
EP2731435(A1) |
申请公布日期 |
2014.05.21 |
申请号 |
EP20130740383 |
申请日期 |
2013.07.08 |
申请人 |
MEIJI SEIKA PHARMA CO., LTD. |
发明人 |
KOBAYASHI, KOEI;SUMIDA, NAOMI;YANAI, KOJI |
分类号 |
C07K14/36;C12N15/113;C12N15/52;C12N15/63;C12N15/76;C12P17/16;C12Q1/68 |
主分类号 |
C07K14/36 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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