| 摘要 |
The 18S PCR method is based on the detection of species-specific small subunit (SSU) rDNA, by amplification of all non-human SSU rDNA in human clinical samples. This is obtained by preferential PCR amplification of non-human DNA extracted from human clinical samples, using three or four different primer sets, high human DNA content samples (steril) and low human DNA content samples (fecal), respectively. The resulting PCR amplicons are then barcoded and sequenced using either sanger or NGS sequencing; single molecule sequencing to circumvent the complications of Sanger sequencing (basically most prevalent amplicon sequencing). This will enable the detection of all amplified non-human eukaryotes in the respective clinical sample. The technique is furthermore compatible with the existing 16S method for detection of all bacteria in clinical samples, in order to provide the most complete detection system of organisms in clinical samples to date. |
