发明名称 |
Method of detecting or quantitatively determining mitochondrial DNA 3243 variation, and kit therefor |
摘要 |
A method for detecting a DNA having the mitochondrial DNA 3243 mutation is disclosed. Quantitative PCR is used with a primer having a nucleotide sequence complementary to the nucleotide sequence starting from the nucleotide number 243 in SEQ ID NO: 2 and having a length of 12 to 30 nucleotides. A method is also disclosed for detecting a DNA having the mitochondrial DNA 3243 mutation by using a nucleic acid probe which is end labeled with a fluorescent dye. The fluorescence of the fluorescent dye decreases upon hybridization. The nucleic acid probe has a nucleotide sequence complementary to the nucleotide sequence starting from nucleotide number 230 in the nucleotide sequence of SEQ ID NO: 2 and a length of 14 to 40 nucleotides. The 3′end of the probe is labeled with the fluorescent dye. |
申请公布号 |
USRE44894(E1) |
申请公布日期 |
2014.05.13 |
申请号 |
US200413234907 |
申请日期 |
2004.04.16 |
申请人 |
HIRAI MITSUHARU;ARKRAY, INC. |
发明人 |
HIRAI MITSUHARU |
分类号 |
C12Q1/68;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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