| 摘要 |
PROBLEM TO BE SOLVED: To identify or determine the quantity with high accuracy without complicating measurement work and photometer operation in fluorescence measurement using antibody reaction.SOLUTION: After a first measurement step in which fluorescent intensity is detected with a detector 3 by irradiating a first inspection liquid 81 containing an antibody labeled with a fluorescent reagent with an excitation light from a light source 1, a second measurement step in which the fluorescent intensity of a liquid phase object 83 obtained by dissolving a sample S that may contain an antibody into a second inspection liquid 82 and then charging the dissolved sample S into the first inspection liquid 81 is detected in the same manner is performed. A fluorescent enhancement ratio Q/Qis obtained by performing arithmetic processing according to the fluorescent intensity value Fin the first measurement step, the fluorescent intensity value Fin the second measurement step, and a correlation ratio R. The correlation ratio R is the ratio between fluorescent intensity to be obtained from an unreactive antibody in the first inspection liquid 81 and fluorescent intensity to be obtained from components other than the unreactive antibody. |
