METHOD OF IMMOBILISING BIOMOLECULES ON SURFACE OF MAGNETICALLY CONTROLLED IRON NANOPARTICLES COATED WITH CARBON COATING

摘要

FIELD: chemistry.SUBSTANCE: invention relates to a method of immobilising protein molecules on the surface of magnetically controlled iron nanoparticles coated with a carbon coating. The method involves reacting powder with 4-carboxybenzene diazonium tosylate dissolved in water to form a covalent bond between organic functional groups and the surface of the powder of iron nanoparticles coated with a carbon coating. Carbodiimide activation is then carried out using the following systems: dicyclohexylcarbodiimide with N-hydroxysuccinimide in dimethyl sulphoxide (DCC/NHS in DMSO) or 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride with N-hydroxysuccinimide in water (EDC/NHS in HO) or a phosphate buffer solution. Covalent linking of protein molecules with the activated COOH group is then carried out in an aqueous or buffer medium.EFFECT: invention enables to immobilise biomolecules on the surface of magnetically controlled iron nanoparticles coated with a carbon coating.3 dwg, 8 ex