METHOD OF MICROCLONAL REPRODUCTION OF BLACK ALDER IN VITRO

摘要

FIELD: biotechnologies.SUBSTANCE: in the method they cultivate callus cultures from sterile explants of stem segments, leaves, leaf stakes. Basic nutrient media are used, such as Murasige-Skuga and Woody Plant Medium with content and ratio of growth regulators: 6-benzylaminopurine - 0.2-1.5 mg/l, ?-naphthyl acetic acid - 0.2-0.5 mg/l, indolebutyric acid - 2 mg/l. Further adaptation is carried out with light of 2000 lux and nutrient mode of regenerants in a greenhouse with production of planting material for forest cultures.EFFECT: method makes it possible to produce planting material with high inherited properties.2 tbl