发明名称 |
Small RNA Capture, Detection and Quantification |
摘要 |
Methods, compositions and kits for capturing, detecting and quantifying mature small RNAs are provided herein. Embodiments of the methods comprise ligating 5' and 3' ligation adaptors to the 5' and 3' ends of the mature small RNAs, respectively, in the presence of 5' and 3' semi-degenerate ligation splints to generate a ligation product. Other embodiments comprise reverse transcribing polyadenylated mature small RNA with a universal reverse transcription primer and ligating an adaptor to the 3' end of the cDNA in the presence of a semi-degenerate ligation splint to generate a cDNA lligation product. |
申请公布号 |
WO2014071322(A1) |
申请公布日期 |
2014.05.08 |
申请号 |
WO2013US68350 |
申请日期 |
2013.11.04 |
申请人 |
LIFE TECHNOLOGIES CORPORATION |
发明人 |
WONG, LINDA;CHEN, CAIFU;WU, YALEI;DONG, SHOULIAN;LIU, CHUNMEI |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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