DNA SEQUENCING BY SYNTHESIS USING RAMAN AND INFRARED SPECTROSCOPY DETECTION

摘要

This invention provides nucleoside triphosphate analogues having the structure: formula (I), wherein B is a base and is adenine, guanine, cytosine, uracil or thymine, wherein R'' is an OH or an H, and wherein R' is azidomethyl, a hydrocarbyl, or a substituted hydrocarbyl, and which has a Raman spectroscopy peak with wavenumber from 2000 cm-1 to 2300 cm-1 or a Fourier transform-infrared spectroscopy spectroscopy peak with wavenumber from 2000 cm-1 to 2300 cm-1, and also to methods of DNA sequencing and SNP detection.