| 摘要 |
Provided herein are gold nanoparticles mediated non-viral delivery of miRNAs, siRNAs, genes and drugs. Nanoparticle platforms and combinatorial drug delivery vehicles comprises gold nanoparticles with a plurality of thilolated hyperbranched dendrons conjugated to the nanoparticle surface. The thiolated hyperbranched dendrons comprise chemically-modifiable surface groups, functionalized interior groups and nano-cavities within the hyperbranched structure to which a variety of payload molecules may be conjugated, optionally via a linker. Payload molecules may comprise nucleic acids, anticancer drugs and small molecule inhibitors, optionally with, non-cytotoxic signaling agents, for example, fluoroscein isothiocyanate. Successful manipulation of the degree of PEGylation and the amount of gold nanoparticles in a polyelectrolyte complex to evaluate the best formulation for highest payload delivery of chemically unmodified miRNA duplexes and stemloops is presented. Also provided are methods for delivering one or more therapeutic agents to a cell or tissue or for treating a pathophysiological condition in a subject by delivering the combinatorial drug delivery vehicles to a cell or tissue associated with the pathophysiological condition to facilitate internalization of the vehicle to effect treatment. |
