A cleavage-based real-time PCR assay method is provided. In general terms, the assay method includes subjecting a reaction mixture comprising a) PCR reagents for amplifying a nucleic acid target, and b) flap cleavage reagents for performing a flap cleavage assay on the amplified nucleic acid target to two sets of thermocycling conditions. No additional reagents are added to the reaction between said first and second sets of cycles and, in each cycle of the second set of cycles, cleavage of a flap probe is measured.
申请公布号
AU2011329376(B2)
申请公布日期
2014.05.08
申请号
AU20110329376
申请日期
2011.11.02
申请人
EXACT SCIENCES CORPORATION
发明人
OLDHAM-HALTOM, REBECCA;ZOU, HONGZHI;LIDGARD, GRAHAM P.;DOMANICO, MICHAEL J.;ALLAWI, HATIM