| 摘要 |
A process of preparing an amino acids concentrate suitable for human intravenous administration comprises subjecting animal serum protein to acid hydrolysis and subsequently treating the amino acid hydrolysate so produced with an ion-exchange resin capable of removing the hydrolysis acid from the hydrolysate, e.g. "Deacidite G or E" (Registered Trade Mark). The animal serum protein is preferably bovine serum protein and the preferred acid is hydrochloric acid. The amino acid hydrolysate is treated with activated carbon before and after treatment with the ion exchange resin. Preferably dry serum protein is refluxed for 6 hours with 12N. HCl and the hydrolysate distilled in vacuo to remove HCl to 4N. and the residue is dissolved in water to a 40% w/v solids content.ALSO:A process of preparing an amino acids concentrate suitable for human intravenous administration comprises subjecting animal serum protein to acid hydrolysis and subsequently treating the amino acid hydrolysate so produced with an ion-exchange resin capable of removing the hydrolysis acid from the hydrolysate, e.g. Deacidite G or E. The animal serum protein is preferably bovine serum protein and the preferred acid is hydrochloric acid. The amino-acid hydrolysate is treated with activated carbon before and after treatment with the ion exchange resin. Preferably dry serum protein is refluxed for 6 hours with 12 N HCl and the hydrolysate distilled in vacuo to remove HCl to 4N and the residue is dissolved in water to a 40% w./v. solids content. A nutrient solution for intravenous injection comprises an amino acid concentrate as above, together with essential aminoacids which have been lost in the process, water-soluble vitamins, a metabolizable sugar, e.g. glucose, sucrose or fructose, ethanol, sodium and potassium chlorides and sterile pyrogen-free water and has a pH of 6.8 to 7.2. A typical solution contains amino acids 4 grams, fructose 12-15 grams, alcohol 3-5 grams, sodium chloride 0.37 grams, potassium chloride 0.335 grams, sodium pantothenate 4 mg., pyridoxine hydrochloride 1.8 mg. and water to 100 ml.
|
