发明名称 |
Organic acid-tolerant microorganisms and uses thereof for producing organic acids |
摘要 |
Organic acid-tolerant microorganisms and methods of using same. The organic acid-tolerant microorganisms comprise modifications that reduce or ablate AcsA activity or AcsA homolog activity. The modifications increase tolerance of the microorganisms to such organic acids as 3-hydroxypropionic acid (3HP), acrylic acid, and propionic acid. Further modifications to the microorganisms such as increasing expression of malonyl-CoA reductase and/or acetyl-CoA carboxylase provide or increase the ability of the microorganisms to produce 3HP. Methods of generating an organic acid with the modified microorganisms are provided. Methods of using acsA or homologs thereof as counter-selectable markers include replacing acsA or homologs thereof in cells with genes of interest and selecting for the cells comprising the genes of interest with amounts of organic acids effective to inhibit growth of cells harboring acsA or the homologs. |
申请公布号 |
US8715973(B1) |
申请公布日期 |
2014.05.06 |
申请号 |
US201313798835 |
申请日期 |
2013.03.13 |
申请人 |
WISCONSIN ALUMNI RESEARCH FOUNDATION |
发明人 |
PFLEGER BRIAN FREDERICK;BEGEMANN MATTHEW BRETT |
分类号 |
C12P7/52;C12N9/88;C12P7/04;C12P7/40;C12P7/42 |
主分类号 |
C12P7/52 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|