发明名称 |
Process for determining the concentration of nucleic acids |
摘要 |
A process is disclosed for determining the concentration of nucleic acids in a sample in a microfluidic device. In at least one embodiment, the method includes a) introducing the sample into a first chamber, b) carrying out a number of cycles of an amplification reaction to be carried out in cycles for amplifying nucleic acids, c) transferring a defined volume which is a fraction of the volume of the first chamber and which has amplified nucleic acids into a second chamber and replacing the transferred defined volume with fresh reagents for the amplification reaction, d) determining the concentration of the amplified nucleic acids in a second chamber equipped with an element to determine concentrations, and e) repeating steps b)-d) until a concentration of the amplified nucleic acids which is within a range is determined in the second chamber. An arrangement is further disclosed. |
申请公布号 |
US8715924(B2) |
申请公布日期 |
2014.05.06 |
申请号 |
US20070808835 |
申请日期 |
2007.06.13 |
申请人 |
GUMBRECHT WALTER;MOSNER JÖRN;SCHMIDT SEBASTIAN;SIEMENS AKTIENGESELLSCHAFT |
发明人 |
GUMBRECHT WALTER;MOSNER JÖRN;SCHMIDT SEBASTIAN |
分类号 |
C12Q1/68;C07H21/04;C12M1/34;C12M3/00;C12P19/34;G01N15/06;G01N21/00 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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