METHOD FOR PROPAGATING STERILE MALE PLANT LINE

摘要

Provided is a method for propagating a sterile male plant line. The method comprises: (a) providing a first plant that comprises a homozygous recessive male sterility allele; (b) providing a second plant comprising the homozygous recessive allele and a construct, where the construct exists in a heterozygous state and comprises i) a first nucleotide sequence that will restore the male fertility of the first plant after expression, and ii) a second nucleotide sequence that is able to affect the grain shape or the endosperm nutrient material composition in a heterozygous state, which allows for distinguishing the grains with or without said construct by observation through naked eyes or devices; the first nucleotide sequence and the second nucleotide sequence are tightly connected with each other and coexist in plants; and (c) fertilizing the female gametes of the first plant with the male gametes of the second plant.