| 摘要 |
To sufficiently remove dependency on distance and the like that remain in an image subjected to dividing operation and to perform observation with fluorescence images that are high in quantitativity. There is provided a fluorescence observation apparatus (1), including: a lighting section (4) including a light source (3) that emits illumination light and excitation light; a fluorescence imaging section (18) adapted to pick up fluorescence generated on a subject (A) and to acquire a fluorescence image (G 2 ); a returned light imaging section (17) adapted to pick up returned light returned from the subject A and to acquire a returned light image (G 1 ); a preprocessing section (23) adapted to multiply at least either the fluorescence image (G 2 ) or the returned light image (G 1 ) by a coefficient in which a distance property of fluorescence intensity and a distance property of returned light intensity, which are acquired in advance with respect to a standard sample, come to be in a relation directly proportional to each other and to generate a fluorescence image for correction (G 2 '') and a returned light image for correction (G 1 ''); and a fluorescence image correction section (24) adapted to divide the fluorescence image for correction (G 2 ''), which was generated by the preprocessing section (23), by the returned light image for correction (G 1 ''). |
