METHODS FOR REDUCING ACCUMULATION OF LACTATE DURING CULTURING AND METHOD FOR PRODUCING POLYPEPTIDE

摘要

The present disclosure relates to methods of decreasing lactate production in cell culture using divalent transitional metallic salts. The present disclosure also relates to a method of producing polypeptide by adding divalent transitional metallic salt to the cell culture medium for reducing lactate accumulation followed by fermenting and recovering the polypeptide.