METHOD OF INCREASING THE EXPRESSION YIELD OF VITAMIN K-DEPENDENT PROTEINS

摘要

The invention encompasses the use of one or more compounds selected from a list comprising i) reduced forms of vitamin K and/or ii) reduced forms of a vitamin K analog and/or iii) reduced forms of a vitamin K precursor for the expression of one or more functional vitamin K-dependent proteins in cell culture as well as processes for the fermentation of eucaryotic cells expressing one or more vitamin K-dependent proteins wherein one or more compounds selected from a list comprising i) reduced forms of vitamin K and/or ii) reduced forms of a vitamin K analog and/or iii) reduced forms of a vitamin K precursor are added to the cell culture medium before and/or during the fermentation process.