摘要 |
<p>Probes and methods are described for detecting polymorphisms, including short tandem repeats, in a target nucleotide sequence. The probes include first and second regions separated by a linker sequence, with the first and second regions having discrete melting temperatures with their respective target sequences. In a first embodiment, the first and second regions are both reporter sequences; in a second embodiment, one region is an anchor sequence while the other is a reporter sequence.</p> |