Focus information generating device and focus information generating method

摘要

It is possible to generate image data of a biological tissue in a short time. A focus information generating device (2) receives a first reflected light beam (Lr1) split from a reflected light beam (Lr) and a second reflected light beam (Lr2) passing through a pin-hole plate (36). A signal processing unit (13) calculates a uniform reflectance (RE) representing a light amount ratio of the second reflected light beam (Lr2) to the first reflected light beam (Lr1) along with a sum signal (SS) and a difference signal (SD). An integrated control unit (11) detects a position (Z1) corresponding to a top surface (104A) of a cover glass (104) on the basis of the sum signal (SS) and the difference signal (SD) and detects a position (Z3) representing a biological tissue (102) on the basis of the sum signal (SS) and the uniform reflectance (RE). As a result, the integrated control unit (11) can calculate a cover distance (DM) from the top surface (104A) of the cover glass (104) to the biological tissue (102) in a pathology glass slide (100) on the basis of the positions (Z1) and (Z3).