METHOD OF DIFFERENTIATING BACILLUS ANTHRACIS FROM OTHER CLOSELY RELATED SPECIES OF GENUS BACILLUS BASED ON DETERMINING DIFFERENCES IN STRUCTURE OF CHROMOSOMAL GENES

摘要

FIELD: biotechnology.SUBSTANCE: method includes sample preparation, DNA isolation, PCR statement. At that in carrying out PCR, the oligonucleotide primers are used, which are complementary to sequences of the chromosomal genes fliC and hom2, having the following sequences: fliC-F: 5'-TGGAGCAGTAACAATTGG-3', fliC-R: 5'-GCACCACTGATAGAAATGTTAG-3', hom2-F: 5'-GACGTGTTAAAAGAAGCCCA-3', hom2-R: 5'-CACCAATTTCGTCTTTTACA-3', followed by electrophoretic analysis of the amplification products, when the formation of the amplification product is 153 bps in size it is indicative of belonging of the strain under study to the species B.anthracis, formation of the amplification product with size of 550 bps is indicative of belonging of the strain under study to the other species of the genus Bacillus.EFFECT: invention enables to differentiate effectively the bacillus anthracis from other species of bacilli.1 dwg, 1 ex