| 摘要 |
FIELD: medicine.SUBSTANCE: invention refers to medicine, namely immunology, and may be used for assessing the functional state of human lymphocytes. That is ensured by assessing 72-hour phytohemagglutinin-stimulated lymphocyte blast-transformation reaction (LBTR) by immunocytochemical method in a luminescence microscope in indirect immunofluorescence test. A Ki-67 nuclear antigen marker is Ki-67 monoclonal antibody. The functional capability of lymphocytes is described by a quantity of visualised (light-producing) Ki-67-positive cells having light emission foci in the cell nucleus visualised after the artificial stimulation to PHA proliferation. If the antigen-positive cell value is less than 49%, the lower functional activity of lymphocytes is stated in the individuals suffering the disordered immune status.EFFECT: using the given method enables the early immune diagnosis, including of oncohematological diseases.4 ex, 1 tbl, 1 dwg |
