| 摘要 |
Disclosed are methods and cellular systems for the generation of predetermined or random alterations at specific genomic location. The invention provides a transient expression and reverse transcription system to generate single-stranded DNA sequences homologous to a target genomic sequence, which can be transported to the nucleus to alter the genetic information of the target genomic sequence. Also provided are cellular and molecular components that can be used to increase the efficiency of the targeted genomic modification process. |
