发明名称 |
AN INDUCIBLE RECONSTITUTION AND REAL-TIME QUANTITATIVE KINETIC SYSTEM FOR THE STUDY OF INTRAMEMBRANE ENZYMES |
摘要 |
<p>The present invention relates to the field of enzymes. More specifically, the present invention provides an inducible reconstitution and real-time quantitative kinetic system for the study of intramembrane enzymes. In a specific embodiment, a method of screening for modulators of an intramembrane protease comprises the steps of (a) contacting in a mixture the protease and a substrate with a lipid under acidic or basic conditions to form a membrane comprising the lipid bilayer, protease and the substrate; (b) contacting a test agent with the membrane mixture; (c) adjusting the pH to physiological conditions; (d) assaying substrate cleavage by the protease; and (e) comparing the assayed substrate cleavage to a reference that does not include the test agent, wherein an increase or a decrease of substrate cleavage by the protease relative to the reference identifies the test agent as a modulator of the intramembrane protease.</p> |
申请公布号 |
WO2014062771(A1) |
申请公布日期 |
2014.04.24 |
申请号 |
WO2013US65186 |
申请日期 |
2013.10.16 |
申请人 |
THE JOHNS HOPKINS UNIVERSITY |
发明人 |
URBAN, SINISA;BAKER, ROSANNA;DICKEY, SETH |
分类号 |
C12Q1/37;C12Q1/34 |
主分类号 |
C12Q1/37 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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