发明名称 |
ADDITION OF AN ADAPTOR BY INVASIVE CLEAVAGE |
摘要 |
This disclosure provides method for adding an adaptor to a genomic sequence by invasive cleavage, as well as a kit for performing the method. In some embodiments, the method comprises: a) hybridizing genomic DNA to an adaptor comprising a double stranded region and a single stranded region comprising a 5′overhang to produce a substrate for a flap endonuclease; b) cleaving the substrate using the flap endonuclease; c) ligating the recessed end of the double stranded region to the fragment to produce an adaptor-ligated DNA; d) intramolecularly ligating the ends of the adaptor-ligated DNA to produce a circular DNA molecule; and e) enzymatically processing the circular DNA molecule using an oligonucleotide that hybridizes to the adaptor and an enzyme. A kit for performing the method is also provided. |
申请公布号 |
US2014113296(A1) |
申请公布日期 |
2014.04.24 |
申请号 |
US201313963723 |
申请日期 |
2013.08.09 |
申请人 |
AGILENT TECHNOLOGIES, INC. |
发明人 |
DAHL FREDRIK;ERICSSON OLOF JOHN;JOHANSSON HENRIK;SRINIVASAN MAITHREYAN |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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