METHOD FOR SYNTHESIZING COMPLEMENTARY DNA

摘要

Provided is a method which enables the highly efficient reverse transcription of a double-stranded RNA virus and also enables the amplification of the resultant reverse transcript. A method for synthesizing complementary DNA using double-stranded RNA as a template, said method being characterized in that a mixed solution of double-stranded RNA extracted from a sample and a primer is subjected to steps (1) and (2) as mentioned below in this order, and subsequently single-stranded DNA is synthesized from the mixed solution using a reverse transcriptase: (1) a step of heating the mixed solution at a temperature equal to or higher than 95˚C and then rapidly cooling the mixed solution; and (2) a step of heating the mixed solution at a temperature lower than 90˚C and then rapidly cooling the mixed solution.