摘要 |
<p>Objects are to provide nucleic acid probes, which in the detection of target nucleic acids with fluorescent quenching probes, can assay a greater variety of target nucleic acids at the same time while suppressing increases in assay labor and cost, and also a method for assaying target nucleic acids by using the nucleic acid probes. Provided is a nucleic acid probe having a base sequence hybridizable with a target nucleic acid sequence in a target nucleic acid. At least one terminal base is labeled with a fluorescence quenching dye, the base labeled with the fluorescence quenching dye is cytosine, the base sequence is designed such that upon hybridization of the nucleic acid probe with the target nucleic acid sequence, at least one guanine base exists in a range of five bases centering at the base in the target nucleic acid sequence, the base being to be paired with the cytosine base, and the fluorescence quenching dye is a fluorescence quenching dye selected from the group consisting of ATTO465, ATTO655, ATT0680 and ATTO700.</p> |