发明名称 |
Method for the co-detection of catechins and caffeine in biospecimens |
摘要 |
PURPOSE: A method for simultaneously analyzing catechins and caffeine in biospecimens is provided to separate and analyze effective materials in specimens collected from livestocks ingesting green tea components. CONSTITUTION: A method for simultaneously analyzing catechins and caffeine in biospecimens includes the following steps: specimens are collected from a target livestock; the specimens are mixed with ascorbic acid-EDTA; the mixture is homogenized using a methanol/ethyl acetate solution and 0.3M sodium dithionite/0.1% Na2EDTA solution; the homogenized solution is concentrated to be applied to a hydrophile-lipophile balance(HLB) column; caffeine is extracted from the solution through the HLB column; and catechin is extracted from the solution using ascorbic acid-EDTA and a methanol/ethyl acetate solution. |
申请公布号 |
KR101387872(B1) |
申请公布日期 |
2014.04.22 |
申请号 |
KR20120050419 |
申请日期 |
2012.05.11 |
申请人 |
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发明人 |
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分类号 |
G01N1/28;G01N30/02;G01N33/08;G01N33/12 |
主分类号 |
G01N1/28 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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