| 摘要 |
PROBLEM TO BE SOLVED: To provide novel ion-exchange media and related methods for their preparation and use.SOLUTION: Ion-exchange stationary phases according to the invention are suitable for chromatographic separation of a variety of biomolecules. Distinguishing characteristics of ion-exchange media according to this invention include, for example, their ability to separate variants of monoclonal antibodies via cation-exchange liquid chromatography using porous substrates with particle sizes of less than 5 μm. The ion-exchange stationary media include a hydrolytically stable layer, which inhibits surface degradation of the particles in 100% aqueous media. Another unique feature is low molecular weight building blocks used to functionalize the particles with ion-exchange groups. |
