摘要 |
The present invention relates to a method for normalizing the contents of biomolecules in a sample, comprising the following steps: a) preparing a reaction vessel with a vessel surface that is functionalized at least in sections–preferably on the inside of the vessel–in such a way that the surface can reversibly bind biomolecules under high salt conditions, b) executing at least one sample preparation step, c) binding biomolecules from the prepared sample to the vessel surface (“binding and normalizing step”) under high salt conditions, d) optionally washing (“washing step”), and e) executing at least one subsequent reaction. The application also relates to a reaction vessel as is used in the above method. |