发明名称 |
COMPOSITIONS, METHODS AND RELATED USES FOR CLEAVING MODIFIED DNA |
摘要 |
Compositions, methods and related uses are provided relating to cleaving modified DNA. For example, a set of DNA fragments obtainable by enzymatic cleavage of a large DNA is described where at least 50% are similarly sized and have a centrally positioned modified nucleotide. In addition, an enzyme preparation is provided that includes one or more enzymes that recognize a modified nucleotide in a DNA and cleave the DNA at a site that is at a non-random distance from the modified nucleotide. The one or more enzymes are further characterized by an N-terminal conserved domain with greater than 90% amino acid sequence homology to WXD(X)10YXGD. The related uses include creating a methylome, methods of purifying DNA fragments containing a modified nucleotide and diagnostic applications. |
申请公布号 |
US2014106350(A1) |
申请公布日期 |
2014.04.17 |
申请号 |
US201313969418 |
申请日期 |
2013.08.16 |
申请人 |
NEW ENGLAND BIOLABS, INC. |
发明人 |
ZHENG YU;ROBERTS RICHARD J. |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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