| 摘要 |
The present invention describes a method of enzyme conversion comprising thesteps of: immobilising an enzyme composition on a support material; drying the support material and enzyme composition to form a solid phase immobilised enzyme system;contacting the system with a gaseous reagent; and allowing the enzyme system to convert the reagent to a product; wherein the enzyme composition comprises a first enzyme, a second enzyme and a co-factor which may be converted between first and second states; wherein the co-factor in the first state promotes reaction of the first enzyme; and wherein the co-factor in the second state promotes reaction of the second enzyme. In an exemplified embodiment, the first enzyme is methane mono-oxygenase, and the second enzyme is alcohol dehydrogenase (ADH), formaldehyde dehydrogenase (FADH), or formate dehydrogenase (FODH), and the co-factor is NAD(H) or NADP(H). In this embodiment the substrate is methane, and can be converted to methanol and ultimately formic acid. |
