发明名称 Novel Glutamate Decarboxylase Mutants For Salt-Free Reaction
摘要 The present invention relates to the development of a novel glutamate decarboxylase (GAD) exhibiting activity even at neutral pH, and to a method for producing gamma-aminobutyric acid (GABA) from high-concentration L-glutamate by using a catalyst in the form of the enzyme itself or cells containing the enzyme. More specifically, the novel glutamate decarboxylase variant is produced by adding a histidine tag for cleavage to the amino terminal of wild-type glutamate decarboxylase and eliminating carboxyl-terminal threonine or adding an excess of residual-group alanine, thereby expanding the active pH range towards neutral compared with the wild type, improving also the gamma-aminobutyric acid synthesis efficiency compared with the wild type and exhibiting almost 100% conversion efficiency, and thus a reaction system is developed in which the active pH range is expanded and glutamate is used as a buffering agent as well as constituting the substrate, and salt-free gamma-aminobutyric acid which is harmless to the body can be produced and absorbed.
申请公布号 KR101379585(B1) 申请公布日期 2014.04.14
申请号 KR20110122943 申请日期 2011.11.23
申请人 发明人
分类号 C12N9/88;C12N15/60;C12N15/63;C12P7/52 主分类号 C12N9/88
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