METHODS AND COMPOSITIONS FOR PURIFYING DNA

摘要

Provided are methods and compositions for extracting or purifying a polynucleotide. Further provided are methods and compositions for extracting a lipopolysaccharide from a solution containing a polynucleotide. The methods may comprise contacting a cell or cell supsension with a composition comprising a non-ionic detergent (NID). The methods may comprise contacting a solution containing a polynucleotide with a composition comprising a Hofmeister series salt (HSS), and contacting the resulting solution with a composition comprising a non-ionic detergent (NID).