| 摘要 |
PROBLEM TO BE SOLVED: To provide methods for conducting multi-stage nucleic acid amplification reactions useful for applications requiring rapid amplification of multiple sequences.SOLUTION: The present invention provides methods and apparatus for carrying out multiple amplification reactions in a single reaction chamber by successive cycles of loading reaction mixture, amplifying, and removing spent reaction mixture in a fluidly closed system. In particular, the present invention allows amplification of a plurality of target polynucleotides from a single sample by carrying out under closed-loop control successive amplifications of different target polynucleotides from different portions of the sample. |
