发明名称 |
Device and method for detecting biomolecule |
摘要 |
Disclosed are a method for detecting a biomolecule including: immobilizing a nucleic acid aptamer capable of specifically binding to a biomolecule to be detected on the surface of a bead on which fluorophores are arranged; hybridizing the nucleic acid aptamer with a guard nucleic acid (g-nucleic acid) labeled with a quencher to quench fluorescence; and reacting a sample including the biomolecule to be detected with the nucleic acid aptamer and detecting a fluorescence signal emitted as the biomolecule binds with the nucleic acid aptamer and the g-nucleic acid labeled with the quencher is separated, and a device for detecting a biomolecule for conducting the detection method. The present disclosure allows for effective, convenient and fast detection of the biomolecule to be detected, enables quantitative analysis, and enables detection of even a trace amount of sample. |
申请公布号 |
US8691500(B2) |
申请公布日期 |
2014.04.08 |
申请号 |
US201213560285 |
申请日期 |
2012.07.27 |
申请人 |
KIM SANG KYUNG;WOO KYOUNGJA;CHOI YU RI;KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY |
发明人 |
KIM SANG KYUNG;WOO KYOUNGJA;CHOI YU RI |
分类号 |
C12Q1/68;C07H21/04;C12M1/34;G01N21/64 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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