| 摘要 |
The present invention relates to a concentration gradient generating microfluidic device and a manufacturing method thereof. The method comprises (a) a step of forming multiple channels separated at a constant interval inside and preparing a polymer mold, at a boundary wall, with a communication space the height of which is lower than that of the channel, (b) a step of bonding a glass substrate to the polymer mold prepared in the (a) step by oxygen plasma, (c) a step of heating the polymer mold in which the glass substrate is bonded by the (b) step, (d) a step of injecting an agarose solution filling along the communication space between the boundary walls of the polymer mold and the glass substrate by surface tension, and (e) a step of leaving the polymer mold under room temperature for few minutes and completing a nanopore membrane as the agarose solution is transformed into hydrogel with multiple nanopores when the agarose solution fills along the communication space of the boundary wall in the (d) step. [Reference numerals] (S100) Forming multiple channels separated at a constant interval inside and preparing a polymer mold, at a boundary wall, with a communication space the height of which is lower than that of the channel; (S200) Bonding a glass substrate to the polymer mold prepared by oxygen plasma; (S300) Mounting the polymer mold in which the glass substrate is bonded in a hot plate maintaining a high temperature and heating the same; (S400) Injecting an agarose solution filling along the communication space between the boundary walls of the polymer mold and the glass substrate by surface tension; (S500) Leaving the polymer mold under room temperature for few minutes and completing a nanopore membrane as the agarose solution is transformed into hydrogel with multiple nanopores when the agarose solution fills along the communication space of the boundary wall |
