| 摘要 |
Disclosed in the present invention are monoclonal antibodies specifically recognizing B-Raf mutant proteins and the preparation method and use thereof. The three monoclonal antibodies prepared by the method of the present invention can specifically recognize three mutant proteins, respectively, i.e., B-Raf N581S, B-Raf V600E and B-Raf V600K mutant proteins, and can be used to detect whether there are N581S, V600E and V600K mutations in the B-Raf proteins of the tissues of an animal or a human in order to provide the basis for the diagnosis and treatment of skin cancer and other various cancers clinically. |
