NUCLEOTIDE SEQUENCE MOTIFS DIRECTING NUCLEIC ACID LOCATION TO EXTRACELLULAR VESICLES

摘要

The present invention discloses the use of isolated short sequence motifs capable of directing or packaging regulatory nucleic acids, preferably RNAs, into extracellular vesicles, preferably exosomes. This mechanism is enhanced by the binding of hnRNP family proteins, which are sumoylated,to such nucleic acid. In this sense, sumoylated hnRNPs directs the loading of nucleic acids into EVs through recognition of specific short motifs disclosed in the present invention. Additionally, the present invention discloses recombinant nucleic acids comprising such sequence motifs, EVs in turn comprising these recombinant nucleic acids, as well as the compositions, preferably pharmaceutical compositions comprising either the recombinant nucleic acids or the EVs of the invention. The identification of such motifs is a useful tool for use in genetic engineering and gene therapy.